At Madeinplant, we offer full-service CDMO (Contract Development and Manufacturing Organization) solutions specializing in plant-based platforms.
We enable clients to outsource the development and production of recombinant proteins using an efficient, biosafe, sustainable, and highly scalable system.
We provide infrastructure, technology, and expertise to support every stage of your project.
Tailored services for each development stage:
1. Genetic design and vector construction
We adapt and optimize your protein’s coding sequence for plant expression:
• Codon optimization for Nicotiana benthamiana
• Design of regulatory elements for transient or stable expression
• Choice of purification tags or signal peptides as needed
• Construction in plasmid or viral vectors
We can work from your existing sequence or handle complete design from scratch.
2. Plant production at laboratory scale
Following design, we perform transient expression of the protein using vector systems derived from plant viruses:
• Controlled cultivation in greenhouse conditions
• Expression monitoring via rapid assays (SDS-PAGE, Western blot)
• Optimization of bacterial OD, post-inoculation timing and environmental conditions
This step allows expression validation before scaling up.
3. Scale-up and pilot-scale production
Once expression is validated, we scale up to pilot production:
• Batch cultivation of Nicotiana benthamiana in greenhouse
• Large-volume inoculation using pre-industrial equipment
• Yield optimization of yields per plant and per square meter
• Biomass harvesting and processing
Our model enables fast transition from lab to pilot scale in just a few weeks.
4. Extraction, purification and characterization
We apply tailored protocols depending on the protein type.
• Plant lysis and extraction with custom buffers
• Clarification, filtration and concentration
• Purification via chromatography (IMAC, IEX, SEC, etc.)
• Characterization of purity, concentration and functional activity
Optional tests available upon request (ELISA, endotoxins, enzymatic activity, etc.).
5. Formulation, freeze-drying and preservation
We deliver your product in the format best suited to its application:
• Stabilized aqueous solution
• Lyophilized powder
• Ready-to-use preparations for cosmetics or in vitro assays
Our protocols are adapted to preserve protein activity and long-term stability, tailored to each molecule.
Flexible collaboration for any pipeline
We offer both turnkey solutions (design – production – purification) and modular services, depending on the needs of your project and stage of development.
Advantages of our plant-based platform
Our system is based on Nicotiana benthamiana, ideal for fast, safe, and scalable production of recombinant proteins, without cell lines or fermenters.
Innovation in bioproduction
- Using plant viral vectors to boost expression yields
- Simultaneous expression of multiple recombinant proteins
- Spatiotemporal expression control via synthetic gene circuits
Our biofactories: the plants
We use Nicotiana tabacum and Nicotiana benthamiana due to their fast growth, easy cultivation, and high protein productivity.
We also develop optimized varieties for enhanced biomass, biosafety, and specific glycosylation patterns.
Feature
Added Value
Growth cycle ≤ 6 weeks
On-demand production, reduced inventory
Low resource consumption
–90% water and energy use vs. microbial fermenters
Glycosylation modulation
“Human-like” protein profiles
Optimized plant lines
High biomass, low alkaloids, high protein yield
Feature and added value
Growth cycle ≤ 6 weeks
On-demand production, reduced inventory
Low resource consumption
–90% water and energy use vs. microbial fermenters
Glycosylation modulation
“Human-like” protein profiles
Optimized plant lines
High biomass, low alkaloids, high protein yield
ADDITIONAL AREAS
Expert plant biodesigners specialized in reprogramming plants to improve their agronomic traits or turn them into valuable biofactories.
INCUBATOR
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